anti-caspase-11  (Novus Biologicals)

Journal: Stem Cell Research & Therapy

Article Title: Loss of G-protein coupled receptor 68 in hematopoietic tissues enhances long-term hematopoietic stem cell function upon aging

doi: 10.1186/s13287-025-04506-z

Figure Lengend Snippet: Gpr68 inhibition reduces activated caspases in LT-HSC. A The activated Caspases of HSPC subpopulations in old C57BL/6 mice compared with young C57BL/6 mice (n = 4). B The Ca 2+ levels of young LT-HSC were treated with Ogremorphin or solvents and old LT-HSC were treated with Ogerin or solvents for 24 h (n = 6). C The activated Caspase 3/7 of young LT-HSC was treated with Ogremorphin or solvents and old LT-HSC was treated with Ogerin or solvents for 24 h (n = 6). D The Gpr68 expression, Ca 2+ levels, and activated Caspase 3/7 of young LT-HSC treated with Ogerin for 24 h (n = 6). E The Gpr68 expression, Ca 2+ levels, and activated Caspase 3/7 of young LT-HSC treated with Ogerin and/or culture upon hypoxia for 48 h (n = 6). F , G The proportion of LT-HSC of LSK cells treated with Ogerin and/or culture upon hypoxia (n = 6)

Article Snippet: BM cells were harvested and stained for HSPC first, then washed with PBS and stained with CaspGLOWTM Fluorescein Active Caspase Staining Kit (Invitrogen, 88–7003) or CellEventTM Caspase-3/7 Green Staining Kit (Invitrogen, C10704 ) according to the manufacturer’s instruction.

Techniques: Inhibition, Expressing